Coding

Part:BBa_K2449012

Designed by: Malte Skovsager Andersen   Group: iGEM17_SDU-Denmark   (2017-09-28)


cex - optimized for E. coli

Part coding the exo-β-1,4-glucanase, and is optimized for for E.coli. It comes from the organsim Cellulomonas fimi.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 524
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 157
    Illegal NgoMIV site found at 530
    Illegal NgoMIV site found at 1032
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 577
    Illegal SapI.rc site found at 660


[edit]
Categories
Parameters
None