Part:BBa_K2440019

miR-2467 target sequence

It is the target sequence of miR-2467, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-2467 in an Ago2 dependent manner, that is, knockdown of miR-2467 was achieved by transfecting cells with miRNA locker.

Hsa-miR-2467-5p was firstly found in human breast tissue. It may be applied in tumor identity with other miRNAs.¹

By using ChIP-Seq, Genechip and miRNA-Seq techniques, hsa-miR-2467-5p was identified as a NF-κB target miRNA in TNFα-stimulated HeLa Cells.²

Sequence and Features

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Experimental Validation

This part is sequenced as correct after construction.

Reference

1.Identification of new microRNAs in paired normal and tumor breast tissue suggests a dual role for the ERBB2/Her2 gene. Persson H, Kvist A, Rego N, Staaf J, Vallon-Christersson J, Luts L, Loman N, Jonsson G, Naya H, Hoglund M, Borg A, Rovira C Cancer Res. 71:78-86(2011).

2.NF-κB target microRNAs and their target genes in TNFα-stimulated HeLa cells. Zhou F, Wang W, Xing Y, Wang T, Xu X, Wang J.