Coding

Part:BBa_K2431025

Designed by: Asa M. Budnick   Group: iGEM17_NortheasternU-Boston   (2017-10-27)


Methionine Aminopeptidase GTG Expression

Methionine aminopeptidase with 5'UTR designed and tested for cell free expression by Bielefeld-CeBiTec 2015. Includes terminator. Amplified with tailed primers containing a promoter region and then used directly in cell-free expression.

Used for removing n-terminal methionines as post-translational modification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None