Part:BBa_K2408023

ADH1_Miraculin

This is a part improvment from BBa_K1033121 - team Uppsala 2013

Miraculin optimized for S. cerevisae with the ADH1 promoter and histidine tag.

The miraculin glycoprotein, originally produced from the Synsepalum dulcificum plant, is a non-cariogenic substance, with a glycemic index of zero. It is suitable for consumption by diabetics and has the ability to convert sour taste into sweet tast. However, miraculin does not change the taste of the foods / drinks consumed. The mechanism of action of this special protein is still not entirely clear, but it is known that it is triggered in the presence of a low pH.

We added the ADH1 strong promoter at the beginning of the sequence and a histidine tag (his * 6) and terminator at the end.

Theerasilp, S., Hitotsuya, H., Nakajo, S., Nakaya, K., Nakamura, Y., & Kurihara, Y. (1989). Complete amino acid sequence and structure characterization of the taste-modifying protein, miraculin. Journal of Biological Chemistry, 264(12), 6655-6659.‏ ISO 690

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 1110
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 771
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1222
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 1110
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 1110
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 93