Part:BBa_K2408012

HXT7pro

HXT7 Yeast promoter

The HXT7 protein is a high-affinity glucose transporter, member of the major facilitator superfamily, and expressed at high basal levels relative to other HXTs. HXT7 transcription is repressed at high glucose levels and is activated when the glucose in the culture approaches depletion. All hexose transporter proteins Hxt2, 4, 6 and 7 in S. cerevisiae are repressed by high glucose concentrations, and induced when glucose concentration decreases below a certain level. HXT7 seems to bind glucose with the highest affinity among all glucose transporters, and this fact is associated with strong induction at low glucose levels. The HXT7 promoter region is suitable for recombinant protein production in yeast and was compared to other yeast promoters (PTEF1, PADH1, PTPI1, PPGK1, PTDH3 and PPYK1) using lacZ as a reporter gene. Among the aforementioned promoters, PHXT7 found to be the strongest promoter in continuous culture with limited glucose level.

Weinhandl, K., Winkler, M., Glieder, A., & Camattari, A. (2014). Carbon source dependent promoters in yeasts. Microbial cell factories, 13(1), 5.‏

Partow, S., Siewers, V., Bjørn, S., Nielsen, J., & Maury, J. (2010). Characterization of different promoters for designing a new expression vector in Saccharomyces cerevisiae. Yeast, 27(11), 955-964.‏

Ye, L., Berden, J. A., van Dam, K., & Kruckeberg, A. L. (2001). Expression and activity of the Hxt7 high‐affinity hexose transporter of Saccharomyces cerevisiae. Yeast, 18(13), 1257-1267.‏ ISO 690

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]