Coding
STS

Part:BBa_K2408003

Designed by: Yaakov Bulka   Group: iGEM17_Tel-Hai   (2017-08-21)


STS

STS - Stilbene synthase, take coumaryl-CoA and malonyl-CoA and convert them to create resveratrol

Stilbene synthase 6, an enzyme produced by Vitis vinifera (Grape), converts p-Coumaroyl-CoA to trans-resveratrol.
Enzyme activity: 3 malonyl-CoA + p-Coumaroyl-CoA = 4 CoA + trans-resveratrol + 4 CO2
This sequence contains ‘6 His tag’ downstream from the gene, and was optimised for S.cerevisiae using IDT sequence optimisation tool.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 751
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 451


[edit]
Categories
//function/biosynthesis
Parameters
protein