Coding

Part:BBa_K2408001

Designed by: Yaakov Bulka   Group: iGEM17_Tel-Hai   (2017-08-23)


TAL

TAL (tyrosine ammonia-lyase) from Rhodobacter sphaeroides optimized for S. cerevisae

Tyrosine ammonia-lyase catalyzes the deamination of L-tyrosine to form p-coumaric acid and amonia. TAL is the first enzyme in the resveratrol biosynthesis pathway. This sequence contains ‘6 His tag’ downstream from the gene, and was optimised for S.cerevisiae using IDT sequence optimisation tool.


Watts, K. T., Mijts, B. N., Lee, P. C., Manning, A. J., & Schmidt-Dannert, C. (2006). Discovery of a substrate selectivity switch in tyrosine ammonia-lyase, a member of the aromatic amino acid lyase family. Chemistry & biology, 13(12), 1317-1326.‏


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 244
    Illegal PstI site found at 416
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3
    Illegal PstI site found at 244
    Illegal PstI site found at 416
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 453
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 244
    Illegal PstI site found at 416
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 244
    Illegal PstI site found at 416
    Illegal NgoMIV site found at 856
    Illegal NgoMIV site found at 1188
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 116


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