Plasmid_Backbone

Part:BBa_K2377016

Designed by: Zhicheng Lin   Group: iGEM17_Lanzhou   (2017-11-01)


pDP: plasmid backbone for dsRNA production

pDP is a plamid backbone with two reverse T7 promotors and double terminators outside biobrick preffix and suffix. It is an advanced version of L4440 plasmid (more rubust and Biobrick standarlized).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2328
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2334
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2328
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2328
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2328
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2343
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1263


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Categories
Parameters
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