Coding
Part:BBa_K2372005
Designed by: Marcel Chris Conrady Group: iGEM17_Tuebingen (2017-10-26)
GyrBR from Staphylococcus aureus with terminator
Gyrase B (GyrBR) is a type II topoisomerase that usually occurs in Staphylococcus aureus. Our goal was to target a pathogen with the new antibiotic therefore, we used GyrBR from methicillin-resistant Staphylococcus aureus to test the efficiency of our new substance. The function of GyrB is to keep chromosomes in an unwound state by negatively supercoiling closed circular double-stranded DNA in an ATP-dependent manner.
In addition our terminator Bam-Hind (BBa_K1882010) from last years competition (2016) was used.
GyrBR | |
---|---|
Function | relaxes positive supercoiled DNA during transcription and amplification |
Use in | Prokaryotic cells |
RFC standard | RFC 10 |
Backbone | pSB1C3 |
Organism | Staphylococcus aureus |
Source | IDT (DNA synthesis) |
Submitted by | Tuebingen 2017 |
References
http://www.uniprot.org/uniprot/A0JJQ7
http://www.uniprot.org/uniprot/P0AES6
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 511
Illegal AgeI site found at 1459 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1262
[edit]
Categories
Parameters
None |