Coding

Part:BBa_K2372005

Designed by: Marcel Chris Conrady   Group: iGEM17_Tuebingen   (2017-10-26)


GyrBR from Staphylococcus aureus with terminator

Gyrase B (GyrBR) is a type II topoisomerase that usually occurs in Staphylococcus aureus. Our goal was to target a pathogen with the new antibiotic therefore, we used GyrBR from methicillin-resistant Staphylococcus aureus to test the efficiency of our new substance. The function of GyrB is to keep chromosomes in an unwound state by negatively supercoiling closed circular double-stranded DNA in an ATP-dependent manner.
In addition our terminator Bam-Hind (BBa_K1882010) from last years competition (2016) was used.

GyrBR
Function relaxes positive supercoiled DNA during transcription and amplification
Use in Prokaryotic cells
RFC standard RFC 10
Backbone pSB1C3
Organism Staphylococcus aureus
Source IDT (DNA synthesis)
Submitted by Tuebingen 2017



References
http://www.uniprot.org/uniprot/A0JJQ7
http://www.uniprot.org/uniprot/P0AES6

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 511
    Illegal AgeI site found at 1459
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1262


[edit]
Categories
Parameters
None