Coding

Part:BBa_K2372001

Designed by: Marcel Chris Conrady   Group: iGEM17_Tuebingen   (2017-10-26)


CloL - amid synthetase from the Clorobiocin biosynthetic pathway
Background
CloL is a amide synthetase which forms the bonding of the amide bonds linking the 3-amino-4,7-dihydroxycoumarin moiety and related acyl moieties in aminocoumarin antibiotics. The different amide synthetases NovL, CloL, CouL and SimL differ by their substrate specificity, but catalyze the same reaction.

CloL
Function Amide Synthetase (secondary metabolism)
Use in Prokaryotic cells
RFC standard RFC 10
Backbone pSB1C3
Organism Streptomyces roseochromogenus subsp. oscitans
Source Heide lab (University of Tuebingen)
Submitted by Tuebingen 2017



References
Chen, Y., Wendt-Pienkowski, E., Ju, J., Lin, S., Rajski, S. R., & Shen, B. (2010). Characterization of FdmV as an amide synthetase for fredericamycin A biosynthesis in Streptomyces griseus ATCC 43944. J Biol Chem, 285(50), 38853-38860. doi:10.1074/jbc.M110.147744


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 470
    Illegal NgoMIV site found at 627
    Illegal NgoMIV site found at 694
    Illegal AgeI site found at 75
    Illegal AgeI site found at 550
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None