RNA

Part:BBa_K2361003

Designed by: Thijs Ettema   Group: iGEM17_Groningen   (2017-10-10)


pUSP45 tracrRNA

This part consists of the required trans-activating crRNA behind a constitutive lactococcal usp45 promoter. Naturally, tracrRNA is needed togheter with dCas9 and from crRNA:tracrRNA;Cas9 complex when it binds to the spacer array containing the spacer from viral DNA. Then, this complex bind to the original viral DNA to introduce a double strand break. In our system, dCas9 is used instead of Cas9, so this RNA-protein complex will only bind to the target array to result in gene expression interference. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
None