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Sample Discontinued
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Coding

Part:BBa_K2361002

Designed by: Thijs Ettema   Group: iGEM17_Groningen   (2017-10-10)


hCas9 operon

The part contains a CRISPR operon which consists of 4 proteins: hCas9, Cas1, Cas2 and Csn2. This operon originates from the type II CRISPR system. All four proteins are essential for spacer acquisition from the viral DNA into the spacer array. Moreover, hCas9, a hyperactive variant of the Cas9 protein, is involved in both spacer selection/cleaving and spacer incorporation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1124
    Illegal NheI site found at 4774
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3403
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//function/crispr/cas9
Parameters
biology
protein