Composite

Part:BBa_K2335003

Designed by: zhi ling lui   Group: iGEM17_KAIT_JAPAN   (2017-10-25)


GFAPpromoter+HlyD+HlyB+TolC+IL-12+HlyA

A part to operate cure allergic rhinitis system. STAT1 is phosphorylated and a dimer was formed in the Part(BBa_K2335005). And when STAT1 binds with GFAP promoter, transcription of the downstream genes begins. IL-12 is present in downstream. IL-12 in downstream is important for the operation of this system. Then the HlyA signal peptide is added to IL-12 produced by IL-12 to let it be secreted out of the cell. And for HlyD, HlyB and TolC, they are gene groups that form membrane protein to carry HlyA signal peptide.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 979
    Illegal NotI site found at 814
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1516
    Illegal BamHI site found at 4339
    Illegal BamHI site found at 5363
    Illegal XhoI site found at 3102
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4451
    Illegal AgeI site found at 3622
    Illegal AgeI site found at 3952
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2040
    Illegal BsaI site found at 5215
    Illegal BsaI.rc site found at 1498
    Illegal BsaI.rc site found at 2413
    Illegal BsaI.rc site found at 2908
    Illegal SapI site found at 2840
    Illegal SapI.rc site found at 2212


[edit]
Categories
Parameters
None