Composite

Part:BBa_K2335001

Designed by: zhi ling lui   Group: iGEM17_KAIT_JAPAN   (2017-10-25)


GFAPpromoter+HlyD+HlyB+TolC+HylA+GFP

A part to check the operation of cure allergic rhinitis system. In order to check if this system is working properly, IL-12 in part BBa_K2335004 was exchanged with GFP. By exchanging IL-12 with GFP, we can check if IL-12 is transported out of the cell by observing the expression of GFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 979
    Illegal NotI site found at 814
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1516
    Illegal BamHI site found at 4339
    Illegal BamHI site found at 5363
    Illegal XhoI site found at 3102
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4451
    Illegal AgeI site found at 3622
    Illegal AgeI site found at 3952
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2040
    Illegal BsaI site found at 5215
    Illegal BsaI.rc site found at 1498
    Illegal BsaI.rc site found at 2413
    Illegal BsaI.rc site found at 2908
    Illegal BsaI.rc site found at 7352
    Illegal SapI site found at 2840
    Illegal SapI.rc site found at 2212


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