Coding

Part:BBa_K2301000

Designed by: Aaron Renberg   Group: iGEM17_Michigan   (2017-10-27)


T4 Holin optimized for E. coli expression

T4 holin is a protein isolated from the T4 bacteriophage that lyses a bacterial cell from the inside. It opens pores in the cell membrane and wall, allowing lysozymes to degrade it. The bacteria dies as it is exposed to the extracellular environment. We incorporated this part along with BBa_K2301001, BBa_K2301002 and BBa_K2301003 into a temperature controlled kill switch for bacteria.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None