Coding

Part:BBa_K2280003

Designed by: Jiaxing Yang   Group: iGEM17_NKU_China   (2017-10-26)


recombinant enzymes in the E. coli fimbriae (Fim) phase variation system

In E. coli, the expression of the fimbriae component, FimA, is controlled in a binary fashion through the inversion of a 314 bp segment of DNA (fimS) that contains the fimA promoter. The inversion of fimS is performed by the DNA recombinase FimE, which binds to two inverted repeat sequences (Inverted Repeat Left and Right, IRL and IRR, respectively) that flank the fimS element. FimE has different binding affinities for IRL and IRR depending on the orientation of fimS, and as a result FimE is able to efficiently cause recombination only when the promoter faces IRR. Therefore, switch inversion is permanent and heritable.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 22
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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