Coding

Part:BBa_K2280000

Designed by: Zixi Yin   Group: iGEM17_NKU_China   (2017-10-26)


Lyase

This part is a lyase gene. Lyase is an enzyme that catalyzes the breaking of various chemical bonds by means other than hydrolysis and oxidation.When the lyase gene expresses, the bacterium will die, which will avoid the diffusion of bacterium to the environment.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 103


Functional Parameters


Function Test

We synthesized the lyase gene whose sequence from Xanthomonas oryzae bacteriophage Xp10 and we transformed it into Enterobacter sp. FY-07. After we add the inducer (arabinose), it is obvious that the bacteria begin to die.

Figure 1Verification of lyase gene function
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Categories
Parameters
None