Part:BBa_K2258000

pL and Pleft Hybrid promoter

Acts as shuttle promoter, since pL from Lambda phage allows expression in Escherichia coli and Pleft from Mycobacterio phage allows expression in Mycobacterium.

Usage and Biology

Acts as shuttle promoter, since pL from Lambda phage allows expression in Escherichia coli and Pleft from Mycobacterio phage allows expression in Mycobacterium.

Reference

1. PL Expression System : A Prokaryotic Expression System, Invitrogen manual
2. Transcriptional regulation of repressor synthesis in mycobacteriophage L5, Chadd E. Nesbit, Margaret E. Levin, Mary K. Donnelly-Wu and Graham F. HatfuU

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]