Coding

Part:BBa_K2238002

Designed by: Bryan Wilkins   Group: iGEM17_ManhattanCol_Bronx   (2017-10-21)


Glucose oxidase (A. niger) Cysteine tagged

Coding sequence for the Aspergillus niger glucose oxidase enzyme which is a reductase that catalyzes the oxidation of D-glucose to D-glucono-1,5-lactone and hydrogen peroxide. This part contains the E. coli codon optimized sequence for the A. niger wild type glucose oxidase. It requires the cofactor, flavin adenine dinucleotide (FAD). FAD acts as the initial electron acceptor, in a redox reaction, and is reduced to FADH2. Then FADH2 is oxidized by molecular oxygen, which is then reduced to hydrogen peroxide.

This variant has a modified 3X-cysteine C-terminal.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 36
    Illegal BamHI site found at 346
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 370
    Illegal BsaI.rc site found at 1588


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Categories
Parameters
None