Part:BBa_K2220033
Express FliC(XynA) fusion protein
This part can express FliC(XynA) fusion protein with the chasis S.cerevisiae.
The flagellar filament is a helical structure composed of subunits of flagellin. And among various flagellin, the FilC has its own unique feature. The N- and C-terminal region of the FliC are important for filament formation, which are the D0 and D1 domain. While the D3 domain which is a central region of the flic playing no role in filament formation. Additionally, when FliC proteins are in the polymeric state, the D3 domain can be exposed on to the surface. So from above, we can replace the central region of flagellin by other proteins without destroying self-assembling ability and if we fuse the enzyme and FliC, we can obtain a filamentous material with high surface density of catalytic sites.
XynA gene can express xylanase A from B.subtilis.
We use XynA gene to replace the central region of FliC gene, so that the xylanase A can replace the D3 domain of flagellin. So it can express FliC(XynA) with the chasis S.cerevisiae.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 712
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1416
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 301
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1116
Illegal SapI.rc site found at 1170
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