Coding

Part:BBa_K2220027

Designed by: Nan Rong   Group: iGEM17_BNU-China   (2017-10-22)


Express beta-tubulin-mGFP fusion protein

This part can express mGFP marked β-tubulin with the chasis S.cerevisiae.

α- and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Microtubules function in many essential cellular processes, including mitosis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
    Illegal BamHI site found at 1031
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2128
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2110
    Illegal BsaI.rc site found at 592
    Illegal BsaI.rc site found at 1994
    Illegal SapI site found at 1128


[edit]
Categories
Parameters
None