Part:BBa_K2220025

Secrete beta-tubulin-mGFP fusion protein

This part can secrete mGFP marked β-tubulin with the chasis S.cerevisiae.

α-Factor is a 13-residue peptide, secreted by cells of α mating type, that acts on cells of the opposite mating type to promote efficient conjugation between the two cell types, which leads to the formation of aα diploid cells. α-factor is synthesized as a precursor of 165 amino acids containing an 83-residue leader and four α-factor coding regions, each preceded by a short spacer peptide.As a natural signal peptide that is widely present in yeast, α-factor has strong and potent secretory properties. Studies have shown that its secretion is almost 100%, which can prevent the secretion of exogenous tubulin from interrupting the yeast's inherent microtubule system.

α- and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Microtubules function in many essential cellular processes, including mitosis.

This part can express α-factor and β-tubulin-mGFP fusion protein, which can secrete mGFP marked α-tubulin.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 286
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 542
  Illegal BamHI site found at 1325
  Illegal XhoI site found at 244
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 2422
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2404
  Illegal BsaI.rc site found at 886
  Illegal BsaI.rc site found at 2288
  Illegal SapI site found at 1422