Part:BBa_K2220024
Secrete mCherry-alpha-tubulin fusion protein
This part can secrete mCherry RFP marked α-tubulin with the chasis S.cerevisiae.
α-Factor is a 13-residue peptide, secreted by cells of α mating type, that acts on cells of the opposite mating type to promote efficient conjugation between the two cell types, which leads to the formation of aα diploid cells. α-factor is synthesized as a precursor of 165 amino acids containing an 83-residue leader and four α-factor coding regions, each preceded by a short spacer peptide.As a natural signal peptide that is widely present in yeast, α-factor has strong and potent secretory properties. Studies have shown that its secretion is almost 100%, which can prevent the secretion of exogenous tubulin from interrupting the yeast's inherent microtubule system.
mCherry is a monomeric fluorescent construct with peak excitation/emission at 587 nm/610 nm, respectively. It is resistant to photobleaching and is stable. It matures quickly, with a t0.5 of 15 minutes, allowing it to be visualised soon after translation.
α- and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Microtubules function in many essential cellular processes, including mitosis.
This part can express α-factor and mCherry-α-tubulin fusion protein, which can secrete mCherry RFP marked α-tubulin.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 286
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 244
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2440
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1414
Illegal BsaI site found at 2422
Illegal BsaI.rc site found at 2120
Illegal SapI site found at 1278
| None |