Device

Part:BBa_K2205006

Designed by: Marcia Pryce, Michael White   Group: iGEM17_Newcastle   (2017-09-21)


AraC/pBad-Fim Standby Switch

pBAD/AraC promoter upstream of the Fim switch part (K2205005). The arabinose inducible promoter pBAD/AraC allows for controlled, titratable expression of the fimE gene to test its function and usability as part of the Sensynova framework.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None