Composite

Part:BBa_K2201028

Designed by: Lennard Karsten   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)


Repair template for HDR-mediated integration of cas9 into E. coli BL21(DE3)

type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2594
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2594
    Illegal NheI site found at 2353
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2594
    Illegal BglII site found at 715
    Illegal BamHI site found at 4632
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2594
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2594
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None