Composite

Part:BBa_K2082252

Designed by: Pascal Schmidt   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-14)


RFP under the control of an optimized lacZ promoter with lambda cI binding site combined with SH2:cI

RFP under the control of an optimized PlacZ with a lambda cI binding site combined with a generator of the fusion protein SH2:cI(lambda)- K2082252

For this part, the SH2:cI(434) fusion protein was modified by canging the cI(434) binding domain to cI of the phage lambda. The sequence for the lambda cI binding domain was taken from the part BBa_K105004. Therefore, the 434 cI binding site was also changed to the specific lambda cI binding site (BBa_K105021 for specific binding of the cI(lambda) repressor protein. Due to time issures it was not possible to did a total characterization of this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 850
    Illegal NheI site found at 873
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 665
    Illegal AgeI site found at 777
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None