Coding

Part:BBa_K2082107

Designed by: Carsten Hain   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-13)


Wild-type polymerase I

Wild-type polymerase I E. coli polymerase I. This enzymes can be used as a control when experimenting with the error prone polymerase I (BBa_K2082106).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 708
    Illegal XhoI site found at 1206
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 108
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1671



The mutation rate of wild type polymerase I was determined as a baseline to the error prone polymerase I by high-throughput sequencing. See BBa_K2082106 for more information.


Figure 1: Determined mutation rate by high-througput sequencing. Expression of wild type polymerase I in E. coli JS200 leads to the basal mutation rate of wild type E. coli (control).

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