Protein_Domain

Part:BBa_K2082007

Designed by: Mikail Sahin   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-14)


Zika E-Protein

Zika E Protein

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1134
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 808
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1503

With this part an E. coli codon optimized E protein of the Zika virus (ZIKV) strain Mr 766 is made available for the use in this competition. It was designed after the construct took from Uniprot (A0A060H177).




Figure 1: Gelelectrophoresis of the ZIKV E Protein. After cloning and PCR-amplification with specific primers the sample was loaded onto an 1% agarose gel. Order from left to right: Sample with ZIKV E protein codon, 1kb DNA Ladder (by New England Biolabs)

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