Regulatory

Part:BBa_K2066126

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-14)


Synthetic Enhancer Project: NRII2302 Upstream Regulatory Region with Promoter

This is the upstream region of NRII2302 mutant coding region in the pACT Tet plasmid from Amit et. al. It contains the promoter that drives the expression of NRII2302, a mutated protein that only has kinase activity that allows it to phosphorylate the NRI protein to let it activate and bind to the enhancer region of the synthetic enhancer circuit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None