Composite

Part:BBa_K2066112

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-14)


Synthetic Enhancer Project: Ntr promoter driven NRII2302 (mut) on UNS Standard

This part is a constitutively expressed NRII mutant which lacks phosphatase activity. This is used to ensure that the only factor effecting the probability of activation of the synthetic enhancer circuit is the amount of bound repressor. This part was used in combination with a synthetic enhancer, either on a separate plasmid or in the same plasmid. [http://2016.igem.org/Team:William_and_Mary/Synthetic_Enhancer synthetic enhancer]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1024
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None