Composite
Part:BBa_K2066112
Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss Group: iGEM16_William_and_Mary (2016-10-14)
Synthetic Enhancer Project: Ntr promoter driven NRII2302 (mut) on UNS Standard
This part is a constitutively expressed NRII mutant which lacks phosphatase activity. This is used to ensure that the only factor effecting the probability of activation of the synthetic enhancer circuit is the amount of bound repressor. This part was used in combination with a synthetic enhancer, either on a separate plasmid or in the same plasmid. [http://2016.igem.org/Team:William_and_Mary/Synthetic_Enhancer synthetic enhancer]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1024
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |