Composite

Part:BBa_K2066050

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-09)


F30 scaffold / 2x Broccoli

Expresses two dimeric Broccoli aptamers in the F30 scaffold (RNA scaffold). Supposedly allows for characterization of relative abundance of RNA transcripts, as broccoli RNA aptamers fluoresce green, however, this fluorescence is extremely faint.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None