Part:BBa_K1954000
LanA
This entry is the open reading frame for the mutacin 1140 prepropeptide coded for by the lanA gene in Streptococcus mutans JH1140. Mutacin 1140 is type A lantibiotic (a linear lanthionine-containing antimicrobial peptide) differing from other bacteriocins by the presence of distinct post-translational modifications.
The mature mutacin peptide is a 22 amino acid screw-shaped molecule that interferes with the viability of a broad range of Gram-positive bacteria by means of membrane perturbation leading to membrane disintegration. In order for mutacin 1140 to be functional the action of modifying enzymes is required. These enzymes catalyze the formation of stereospecific thioether bridges, dehydration, decarboxylation of the core peptide and leader peptide cleavage, as well as act as exporters of the modified mutacin. In the host organism, 10 of the 22 amino acids are modified to lanthionine, methyllanthionine, 2,3-didehydroalanine, 2,3-didehydrobutyrine and possibly S-(2-aminovinyl)-D-cysteine.
This CDS could be used in a first step towards the assembly of a bacteriocin-producing device. Such a device could be employed as an e.g. anti-cariogenic strategy.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 57
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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