Coding

Part:BBa_K1950006

Designed by: Kyohei Takekawa   Group: iGEM16_Nagahama   (2016-10-14)


ispC-ribB(G108S) fusion

This part is a translation fusion between ispC and ribB G108S mutant. They are linked by linker G2 (GSGGSG). RibB G108S enzyme synthesize 1-Deoxy-D-Xylulose 5-Phosphate (DXP) from ribulose 5-phosphate. ispC enzyme synthesize 2C-methyl-D-erythritol 4-phosphate (MEP) from DXP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1599
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 441
    Illegal AgeI site found at 1489
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None