Part:BBa_K1860702
constitutive promoter and miRNA2911
miRNA2911 Lonicera japonica or the Japanese honeysuckle plant is a well-known Chinese herb and has been used to effectively treat influenza infection for thousands of years. The group around Zhou found that the major portion of detected miRNAs in this plant are being degraded by boiling and only miRNA2911 stayed resistant and active in a decoction. Throughout a bioinformatival prediction and luciferase reporter assays they showed that miRNA2911 has antiviral properties by targeting various Influenza A viruses (IAV); e.g. H1N1, H5N1, H7N9. miRNA2911 is highly stable because of its folding and elevated levels could be detected in peripheral blood and lung tissues of mice after gavage feeding of the honeysuckle decoction.
Usage and Biology
BioBrick state: We submitted the constitutive promoter and miRNA2911 BioBrick to the registry. No sequencing validation was done yet, but an unspecific insert validation via colony PCR was successful.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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