Part:BBa_K1796010

nifE promoted from Paenibacillus sp. WLY78

Function: scaffold gene of nitrogen fixatiion gene cluster,encode FeMo-cofactor assembly protein.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1369
  Illegal PstI site found at 1015
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1369
  Illegal PstI site found at 1015
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1369
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1369
  Illegal PstI site found at 1015
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1369
  Illegal PstI site found at 1015
  Illegal NgoMIV site found at 360
  Illegal NgoMIV site found at 607
  
• 1000
  COMPATIBLE WITH RFC[1000]

Parameter of Protein

Number of amino acids: 452

Molecular weight: 49505.3

Theoretical pI: 6.01

Amino acid composition: 

Ala (A) 39 8.6%

Arg (R) 29 6.4%

Asn (N) 11 2.4%

Asp (D) 19 4.2%

Cys (C) 7 1.5%

Gln (Q) 16 3.5%

Glu (E) 37 8.2%

Gly (G) 45 10.0%

His (H) 10 2.2%

Ile (I) 30 6.6%

Leu (L) 38 8.4%

Lys (K) 21 4.6%

Met (M) 10 2.2%

Phe (F) 12 2.7%

Pro (P) 21 4.6%

Ser (S) 32 7.1%

Thr (T) 24 5.3%

Trp (W) 6 1.3%

Tyr (Y) 15 3.3%

Val (V) 30 6.6%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 56

Total number of positively charged residues (Arg + Lys): 50

Atomic composition:Carbon C 2187

Hydrogen H 3471

Nitrogen N 613

Oxygen O 663

Sulfur S 17

Formula: C2187H3471N613O663S17Total number of atoms: 6951

Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 55725

Abs 0.1% (=1 g/l) 1.126, assuming all pairs of Cys residues form cystines

Ext. coefficient 55350

Abs 0.1% (=1 g/l) 1.118, assuming all Cys residues are reduced

Estimated half-life:The N-terminal of the sequence considered is E (Glu).

The estimated half-life is: 1 hours (mammalian reticulocytes, in vitro).

30 min (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:The instability index (II) is computed to be 40.70

This classifies the protein as unstable.

Aliphatic index: 86.55

Grand average of hydropathicity (GRAVY): -0.239