DNA

Part:BBa_K1791000

Designed by: Graeme Glaister   Group: iGEM15_Lethbridge   (2015-09-17)


Theophylline dependent ribozyme

Theophylline dependent ribozyme with a spacer sequence (AU rich) flanking both sides of the part to assist in proper folding of the ribozyme. The ribozyme has been shown to induce cleave in vivo upon the addition of theophylline. However expressing it in vitro results in cleavage of the ribozyme both with and without the addition of theophylline.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rna/aptazyme
Parameters
None