Composite

Part:BBa_K1628301

Designed by: Xinhao Song   Group: iGEM15_Nankai   (2015-09-05)

P1-GFP

P1-GFP is a composite part used for the Measurement track of this year. This part consists of the promoter part of BBa_J23101, the official GFP device (with an RBS, GFP coding sequence and a set of double terminators) and a pSB1C3 backbone. We transformed this plasmid into E. coli cells and the fluorescence intensity of the GFP protein that was expressed by the bacteria was measured afterwards by a flow cytometry.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


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Categories
Parameters
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