Plasmid_Backbone

Part:BBa_K1473005

Designed by: Shan, Zhe   Group: iGEM14_TCU_Taiwan   (2014-09-17)

Description

Phagemid pBluescript is a special plasmid, it functions as a normal plasmid when transformed into bacteria. But when helper phage M13KO7 infect the bacteria, it will produce progeny M13 phage as vector for pBluscript.
pBluescript contains a f1 ori while M13KO7 helper phage’s f1 ori has been knock-down. So after the infection of M13KO7, its genome can produce protein and replicate but these proteins will package pBluescript as their true “genome”. As a result, the progeny phage’s genome will only contains pBluescript’s MCS, they cannot make next generation.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2908
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2914
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2908
    Illegal XhoI site found at 2896
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2908
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2908
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2923
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 2357
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1380
    Illegal SapI site found at 297
[edit]
Categories
Parameters
n/aphagemid pBluescript