Composite

Part:BBa_K1453006

Designed by: Renhe Luo   Group: iGEM14_SJTU-BioX-Shanghai   (2014-10-17)

TAL USB-His Tag

This part is designed as a scaffold of the second kind of connectee, which can be free in cytoplasm.

Structure

FL-TAL_USB-His_Tag.png

Figure 1 TAL USB-His Tag

In order to bind TAL protein designed by 2012 Freiburg iGEM team, the TAL USB also consists of T1 sequence, T14 sequence and two sites for type II restriction enzyme BsmBI.

When digested with BsmBI, this part can produce two sticky-ends that can bind TAL-Protein DiRepeat (Bba_K747000 to Bba_K747095)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None