Coding
CirCir

Part:BBa_K1392930

Designed by: Burak Kızıl   Group: iGEM14_METU_Turkey   (2014-09-29)


2-hydroxy-6-oxohepta-2,4-dienoate hydrolase

TodF Gene from Pseudomonas putida (F1 Strain) is for reaction of 2-hydroxymuconate semialdehyde + H2O <--> formate + 2-oxopent-4-enoate

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 309
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 309
    Illegal NotI site found at 213
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 309
    Illegal BamHI site found at 266
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 309
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 309
    Illegal NgoMIV site found at 76
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 52


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Categories
Parameters
None