Composite

Part:BBa_K1375022

Designed by: Zheng Xu   Group: iGEM14_SCU-China   (2014-10-10)


promoter+RBS+araC+ DT+pBAD+RBS+Cinr+RBS+CinI+DT+Plac+RBS+LuxI+DT+Pcon+RBS+LacI

This is a long part that linked with k1375031 and k1375032

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1247
    Illegal NheI site found at 1522
    Illegal NheI site found at 4167
    Illegal NheI site found at 4190
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5371
    Illegal BamHI site found at 1187
    Illegal BamHI site found at 1462
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2081
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2589
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None