Reporter

Part:BBa_K1375002

Designed by: Zheng Xu   Group: iGEM14_SCU-China   (2014-10-10)

PRhl/Las+RBS+GFP+DT

This is a reporter biobrick that can be regulated by C4-HSL and C12-HSL. It would express GFP if C4-HSL is added only, but if then C12-HSL is added, the expression would be reduced. Also, to make this reporter work successfully, two coding sequences is needed, RhlR and LasR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 725


[edit]
Categories
Parameters
None