Part:BBa_K1315006

The manA promoter from Xanthomonas campestris

This is the manA gene promoter taken from Xanthomonas campestris pv. campestris. It is activated by the transcriptional activator Clp(BBa_K1315005). Clp is inhibited until RpfG (BBa_K1315003) descreases the levels of cyclic di-GMP after receiving input from RpfC (BBa_K1315002).

Usage and Biology

The DSF-RpfC(BBa_K1315002)-RpfG(BBa_K1315003) sensory system, in all Xanthomonads, activates the production of the manA gene, which codes for endo-mannanase. It has been shown that the transcriptional regulator Clp(BBa_K1315005), when activated binds to the promoter of manA and turns on its synthesis. Clp is usually inactive by cyclic di-GMP molecules that hinder the ability of the transcriptional factor to bind to DNA.

http://www.ncbi.nlm.nih.gov/pubmed/23285129 http://www.ncbi.nlm.nih.gov/pubmed/20073482

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]