Part:BBa_K1315005

The clp gene from Xanthomonas campestris

This is the coding sequence for the cAMP receptor-like protein (Clp). This is a transcriptional activator that is normally inhibited by cyclic di-GMP. Inhibition is alleviated by the RpfG regulator(BBa_K1315003) of the RpfC-RpfG two-component system.

Usage and Biology

Quorum sensing and bacterial cell signalling are mechanisms known to be utilised by bacteria to regulate their virulence. The RpfC-RpfG sensory system has been found to activate the production of virulence factors like extracellular enzymes and polysaccharides in Xanthomonas campestris. It has been also suggested that this system is involved in linking environmental stimuli, including DSF, with pathogenicity in Xanthomonas campestris. DSF is a cis-unsaturated fatty acid from the DSF family. The RpfC-RpfG two-component system is the most well understood one and is present in all Xanthomonads. When RpfC(BBa_K1315003) picks up DSF, it eventually signals RpfG(BBa_K1315003) to degrade cyclic di-GMP. This stops the inhibition of Clp and the transcriptional activator can activate genes.

http://www.ncbi.nlm.nih.gov/pubmed/?term=r+ryan+dsf+family http://www.ncbi.nlm.nih.gov/pubmed/?term=A+two-component+system+involving+an+HD-GYP+domain+protein+links+cell%E2%80%93cell+signalling+to+pathogenicity+gene+expression+in+Xanthomonas+campestris

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 146