Project

Part:BBa_K1230015

Designed by: Andrés Ferriño Iriarte   Group: iGEM13_Ciencias-UNAM   (2013-09-26)


araC-pBad-rbs-LL37-rbs-GFP-pLacI-rbs-MarA Generator

LL-37 antimicrobial peptide with human codon usage under the Arabinose promoter with GFP generator. The MarA protein acts as a transcriptional activator in E. coli. It allows the expression of the acrAB and tolC operons, which activate the AcrAB-TolC efflux pump, a mechanism that has been related with resistance to cationic antimicrobial peptides, such as LL-37. This composite part allows MarA expression in presence of IPTG, by using the LacI promoter and the RBS based on Elowitz repressilator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1164
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 999
    Illegal AgeI site found at 2629
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2028
    Illegal SapI site found at 981


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Categories
Parameters
None