Composite

Part:BBa_K1214013

Designed by: Alec Spinhirne   Group: iGEM13_UT_Dallas   (2013-09-16)

Promoter-RBS-Cra

Constitutive promoter with fructose operon repressor downstream. Should constitutively express Cra (also known as FruR, BBa_K1214008). May be inducible in the presence of Fructose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Biology

The FruR gene encodes a protein which normally binds to and represses pFru promoter. When fructose is present in the system, it binds to the FruR protein and prevents it from repressing the operon promoter.
http://i826.photobucket.com/albums/zz188/lana_khazma/fancy%20stuff/fruRstuff_zpsaf5e8f5b.png" border="0" alt=

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