Regulatory

Part:BBa_K1214006

Designed by: Alec Spinhirne   Group: iGEM13_UT_Dallas   (2013-09-16)

pScr

Sucrose operon promoter. Regulated by sucrose operon repressor ScrR. This was incorporated into part BBa_K1214012.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Biology

The ScrR gene encodes a protein which normally binds to and represses pScr promoter. When sucrose is present in the system, it binds to the ScrR protein and prevents it from repressing the operon promoter.

"http://i826.photobucket.com/albums/zz188/lana_khazma/fancy%20stuff/sucrstuff_zps30aefeaf.png"

Testing

Promoter Strength


Promotion was tested against pFru (BBa_K1214007) and a constitutive promoter (BBa_K823014) using Flow cytometry. All three promoters were ligated in front of RBS-GFP to constitutively express GFP. The cells were inoculated for 18 hours at 37 C in 3 mL of chloramphenicol broth and then examined under fluorescent microscopy.
FDpScrGFP.jpg

Flow cytometry was then completed to determine Mean GFP. FlowPromoters.png
This shows that the pScr promoter strength is comparable to the well-characterized BBa_K823014 promoter.


[edit]
Categories
Parameters
None