Coding

Part:BBa_K1131002

Designed by: Ramya Prathuri   Group: iGEM13_Berkeley   (2013-09-11)


B-Glucosidase; B. Circulans

The beta-Glucosidase from B. Circulans cleaves the glycosidic linkage of our substrate of interest, indican, via hydrolysis. This enzyme is expressed well in E. Coli and shows activity with a variety of substrates including indican and X-Gal, offering a potential alternative in the latter case to the lacZ beta-galactosidase in a blue-white screen. This sequence corresponds to the ORF of the enzyme only.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None