Protein_Domain

Part:BBa_K1085009

Designed by: Claudio Tiecher   Group: iGEM13_Groningen   (2013-09-10)

SilkTail Strep-tag Stop-codon

This BioBrick contains the coding sequence for part of the MaSp2 spider silk protein C terminal domain (SilkTail) together with Strep-tag behind it and ends with a stop codon.

The SilkTail has been optimized in order to match the translation rate between Bacillus subtilis and Argiope aurantia.

The SilkTail is part of the C terminal domain of MaSp2 spider silk.

The Strep-tag, fused at the C terminal, is used both as a binding tag to use to indicate its production and as a binding component to Streptavidin.


Related Biobrick

BBa_K1085008 is the same but it has no Strep-tag behind it.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/bsubtilis
//proteindomain/tail
Parameters
None