Coding

Part:BBa_K1054021

Designed by: Jiasheng Wang   Group: iGEM13_ZJU-China   (2013-09-15)

ssDsbA+beta-lactamase+lgt+streptavidin

This part is used to confirm the localization of membrane scaffold. Beta-lactamase is fused to the periplasmic end of membrane scaffold. If the fusion protein is successfully targeted to the inner-membrane, the bacteria will have ampicillin resistance.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1862
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1664
    Illegal AgeI site found at 1904
    Illegal AgeI site found at 1955
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 723
    Illegal BsaI site found at 1285


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Categories
Parameters
None